12/2/2023 0 Comments 10x chromium read lengthThis results in final 10x libraries that either represent the 3' end of the transcript (as the 10x Barcode is adjacent to the polyA tail on the 3' end of the transcript) or the 5' end of the transcript (as the the 10x Barcode is adjacent to the TSO and the 5' end of the transcript).Ī schematic diagram comparing the final library construct for the two assay schemes is illustrated below. Downstream of fragmentation, only transcripts containing both (1) a 10x Barcode AND (2) an Illumina Read 2 adaptor, which is ligated on to the cDNA after fragmentation, will be amplified during the Sample Index PCR. A template switching oligo (TSO) is used in both workflows to reverse transcribe the full-length transcript.Īfter amplifying the cDNA, molecules are randomly fragmented under conditions that favor 300-400 bp length fragments. Both solutions use polydT primer for reverse transcription, although in the 3' assay the polydT sequence is located on the gel bead oligo, while in the 5' assay the polydT is supplied as an RT primer. Sequencing libraries were loaded at 2.1 pM on an Illumina NextSeq500 with 2 × 75 paired-end kits using the following read length: 98 bp Read1, 14 bp I7 Index, 8 bp I5 Index and 10 bp Read2. For a complete list of cellranger vdj command-line arguments, run cellranger vdj -help. Question: What is the difference between Single Cell 3' and Single Cell 5’ Gene Expression libraries?Īnswer: The two assays are similar but capture different ends of the polyadenylated transcript in the final library. Chromium Next GEM Single Cell 5' Library Gel Bead Kit v1.1 reagent (10X. To generate FASTQ files, refer to the instructions on running cellranger mkfastq.For help getting started, try the cellranger vdj tutorial.
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Sidify Apple Music Converter 4.7.0 Crack + Serial Key With Free Download 2022 12/2/2023 0 Comments Red sparrow bookGenuinely, when Burbank’s elderly team of grandparents where following the Russian resident in Washington, I was rooting for them- probably more than I was for Nash or Dominika at any point in this novel. Chief amongst them is “ ’the Philosopher’ goateed Socrates Burbank, nearly eighty, thrice-married and thrice divorced”. Unlike the FBI, these guys plan in the scale of months rather than days. I really enjoyed Matthews’s descriptions of the ‘retired’ CIA agents sitting on the Russian resident’s tail, trying to predict the way he moves. Is anyone tempted to recreate by the delicious sound of “SVR Cafeteria Chicken Kiev” (do we really think the spy cafeteria is that great at cooking?) or Marta’s “Last Meal- Pytt I Piana“ (eaten just before Marta is sexually abused and murdered?) It got to the point that whenever the characters ate a meal I had to check whether that was the recipe at the end of each chapter. Matthews is trying to make his American readers familiar with Russian cooking.īut no, when the characters travel to Italy or Greece, we get those recipes too. Initially, I thought this was a “cultural introduction to Russia” thing. But to a great extent, it was the BRUSH.īecause clearly, we need something to calm us down among all the sex and violence. Some of it was the vaguely unrealistic circumstance of Dominika’s career. Genuinely, there was a point where I thought I would give up reading this book. When I was writing this blog I had a special Google of antique tortoiseshell brushes, just in case I was missing something crucial about them. To brush the point home, as it were, the evil SVR executioner fantasizes about putting knives in the place the brush usually goes throughout the entire length of the novel. Not least, because regardless of the sexual pleasure the brush gives her at one point - in an entirely furious and non-sexual way - she fantasizes about sticking the same brush into a high ranking SVR official’s ass. We believe that Great-Grandmother’s tortoiseshell brush may be the symbol both of Dominika’s stormy sexuality and her anti-regime resistance. Please note the return to the theme of both literal and metaphorical storms. Dominika trailed the handle down Anya’s stomach, making the soft amber curve infinitely light, infinitely insistent.” “Her great-grandmother’s brush, her mother had brushed her own hair with it, it was her secret companion during the thunderstorms of her girlhood. In Dominika’s lesbian sex scene with a fellow student of the sparrow school: And about the clumsy literal depiction of ‘stormy adolescence’. The author clearly has no idea what he is talking about. Temporary glitch, the author is trying to write a teenage girl. “The swan-necked handle of Prababuschkas brush in her long fingers, timing the lightning flashes to match her own rhythms”. Perhaps the brush will trigger some fond memories.ĭominika tests ”her corporeal boundaries without a thought of shame” A nice description of a sentimental family keepsake. The hog’s hair bristles made a quiet stirring sound, crimson in the air. The tortoiseshell brush with a gently curved handle that, along with a framed photograph and a silver samovar, was the only family belonging rescued from the elegant house in pre-Bolshevik Petersburg. “With the long-handled brush that belonged to her great-grandmother. If that were all this novel was, it would be fantastic.īut then, for some reason we have to have:įirst, we have a description of Dominika’s mother brushing her hair A double-agent will discuss state secrets with his handler in a hired hotel room until the late hours of the morning. The novel is an impressive depiction of intelligence tradecraft – with spies winding their way across Washington and Moscow, losing their pursuit and depositing their secrets at hidden drop-points. And maybe, just maybe, Nate Nash is her chance to fight back… But Dominika was blackmailed into joining the SVR in the first place. Someone has been passing valuable information to the CIA.ĭominika, an SVR agent, is tasked with seducing Nate Nash, whom the SVR suspects of being the mole’s handler. There is a mole-hunt on-going in the SVR (the agency that inherited the legacy of the KGB in Russia). The book had very enthusiastic reviews – and was advertised as in “the tradition of John Le Carré”. The author, Jason Matthews is a retired CIA agent. The movie had scathing reviews, but the rumour said the book was quite good. I heard of the book when the movie came out a few years ago. 12/2/2023 0 Comments Abandoned houses for saleThe compilation of Listings and each individual Listing are Copyright 2023 Central Virginia Regional Multiple Listing Service, LLC. All CVR MLS information provided is deemed reliable but is not guaranteed accurate. All or a portion of the multiple Listing information is provided by the Central Virginia Regional Multiple Listing Service, LLC, from a copyrighted compilation of Listings. The Detroit Land Bank Authority owns vacant residential properties, including homes, side lots, and vacant land that may be developed. Information is provided for consumers' personal, non-commercial use, and may not be used for any purpose other than the identification of potential properties for purchase. Information last updated on, 5:34:55 PM. Information is reliable but not guaranteed. First, just the facade of the house is eerie, yet some people call it gorgeous. Some properties that appear on the website may no longer be for sale, and may be under contract or sold. This abandoned mansion in Gonzales could be haunted for two reasons. Some properties listed with participating firms do not appear on this website at the request of the seller. Some real estate firms do not participate in IDX and their listings do not appear on this website. This property is offered without respect to any protected classes in accordance with the law. Also For Sale 1 Octant Way - 2 Houses For 235,000 - Both Houses Need Major Renovation Work - Properties Back Up To. See examples of abandoned houses, cottages, and farms in different regions of the UK. Find out how to locate the owner, access local council schemes, and renovate derelict houses and farms. BRIGHT MLS and ZeroDown assume no responsibility for typographical errors, misprints or misinformation. Learn how to buy a distressed property in the UK for less than £10,000. The information on each listing is furnished by the owner and deemed reliable to the best of his/her knowledge, but should be verified by the purchaser. The information included in this listing is provided exclusively for consumers' personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing. 12/2/2023 0 Comments Gladys knight top hitsGladys Knight recorded the theme song for the James Bond film, Licence to Kill in 1989, which became a top ten single in the UK. A list of the top 100 songs of 1977, based on performance on the Billboard Hot 100 chart. The Miracles first recorded the song on Augand included their version on their 1968 album, Special Occasion. Two years later, she released her 1991 album, Good Woman, which included the top five R&B hit, 'Men', and Grammy-nominated duet version of 'Superwoman', recorded with Dionne Warwick and Patti LaBelle. Gladys Knight & the Pips Midnight Train to Georgia (1973) 20. Discography Letter Full of Tears (1962) Gladys Knight and the Pips (1965) Everybody Needs Love (1967) Feelin Bluesy (1968) Silk n Soul (1968) All In A. The Marvin Gaye version was placed on his 1968 album In the Groove, where it gained the attention of radio disc jockeys, and Motown founder Berry Gordy finally agreed to its release as a single in October 1968, when it went to the top of the Billboard Pop Singles chart for seven weeks from December 1968 to January 1969 and became for a time the biggest hit single on the Motown label (Tamla). Soul Northern Buy used: 6.99 FREE delivery March 16 - 21. The Gaye recording has since become an acclaimed soul classic, and in 2004, it was placed 81 on the Rolling Stone list of The 500 Greatest Songs of All Time. On the commemorative fortieth anniversary of the Billboard Hot 100 issue of Billboard magazine in June 2008, Marvin Gaye's "Grapevine" was ranked sixty-fifth.ĭetails Or fastest delivery March 10 - 13. It was also inducted to the Grammy Hall of Fame for "historical, artistic and significant" value. The easy, fast & fun way to learn how to sing: 30DaySinger. 12/1/2023 0 Comments Commodore 64 barney errorMy skills at composing music were minuscule compared to the talents of Ben. He was with me from the very beginning when we first started learning to code back at school, writing educational software. Intertwined with all this games writing, I used to team up with a good friend, Ben Daglish. I also did the Atari version of William, but I have no idea if that got released. I wrote the C64 version of Monty Mole and Black Thunder, then left for Wizard Development where I wrote William Wobbler and Gryphon. I moved on from Alligata to yet another shop software house, this time it was Gremlin Graphics, where I wrote Potty Pigeon towards starting the company. Each game I wrote was an attempt beat the last. I felt like I would never move on from the C64 because it felt like there was so much more to give. I ended up writing more than six games as I went on to write Killer Watt, Loco and Blagger as well. The owner of Alligata Software offered me a C64 and in return I wrote six games. I guess I was lucky as I didn’t have to pay for my C64, not in money anyway. The buzz I got from showing off on this machine got me addicted to wanting more! Luckily it was something I was able to make a living from. "The C64 was the machine that moulded my future. THE C64 40 YEAR CELEBRATION with Antony 'Ratt' Crowther (30/31) What's not to love? I even made enough money out of it to put a deposit on my first house." Read the full interview here » Every day was different, and we were in control of it. It never seemed like a real job, although we still worked nine-to-five because that's what we were used to. Interview quote: "It was a fabulous time, because Steve and I had both taken a risk to do something we were passionate about and loved doing. Andrew is of course famous for programming games like Uridium, Paradroid, Morpheus and Gribbly's Day Out, and we delve into details in this one so I'm 100 percent sure you will enjoy it. As if a C64 40 years celebration text from Antony Crowther today wasn't enough! We're happy to give you an interview with legendary programmer Andrew Braybrook. 12/1/2023 0 Comments Beyond van gogh locations 2022Nevertheless, the magazine Boston expressed that there doesn't seem to be a distinct reason behind the mass of immersive events. The show depicts a Van Gogh-themed experience in Paris. Showings in the United States grew traction following the 2020 Netflix series Emily in Paris. The exhibit was created by Annabelle Mauger, who built off a model her husband's grandfather created, known as "Image Totale". The first showing, in 2008, was titled "Imagine Van Gogh: The Immersive Exhibition". Other artists have also been featured in similar shows, including Picasso and Monet, though Van Gogh's popularity makes his shows the most successful. The first immersive experiences of Van Gogh's art took place in Europe in the 2000s. The show tells van Gogh's story with a loose stream of consciousness, using large images and animations to demonstrate what "flashed before his eyes" before the artist died. The "Immersive Van Gogh Exhibit", designed by Massimiliano Siccardi, is adapted for each space it is exhibited in. There is separately in New York "Van Gogh: The Immersive Exhibition". The original show, "Imagine Van Gogh: The Immersive Exhibition", shows works from the artist's last two years living. Some events involve virtual reality headsets that take visitors through the artist's experiences. Some exhibits also use sensory tools like aromas of cedar, cypress, lemon, or nutmeg to help visitors feel more immersed with the works. The works are typically accompanied by music set to pair with them. Most of the experiences involve guests moving from room to room, with walls (and sometimes floors or ceilings) decorated with moving projections of van Gogh's works. Museums in Columbus, Dallas, Houston, and Santa Barbara are hosting unaffiliated traditional exhibits of van Gogh's works, running over similar periods to the immersive exhibitions. Larger cities like Boston, Dallas, Miami, and New York City have multiple shows through multiple vendors there are about six total. cities, including Atlanta, Chicago, Columbus, Houston, Indianapolis, Los Angeles, Phoenix, San Francisco, Seattle, St. Numerous van Gogh events are being held in mid- to large-size U.S. The for-profit shows range in price from $25 to $75 USD per adult. To accidentally purchase tickets to the wrong event. These events drew complaints over their similar naming, which lead customers Events held in the United States include "Imagine Van Gogh: The Immersive Exhibition", the "Immersive Van Gogh Exhibit", "Van Gogh: The Immersive Experience", "Van Gogh Alive", and "Beyond Van Gogh". The immersive events are being held in North America, Europe, Asia, and the Middle East. The van Gogh immersive experiences have received varying degrees of criticism, including over ticket providers, confusion over similar event names, and over artistic licenses taken to van Gogh's paintings.Įvents Video of a "Van Gogh Alive" exhibit Images or videos of the artist's works are projected onto walls, ceilings, and floors, sometimes accompanied by animations, narrations, music, or fragrances. The events are typically set up in large gallery spaces. The for-profit events range across venues, organizers, and cities around the world, though the majority have been held in North America in 20. Type of art exhibit "Van Gogh Alive" exhibit in Wellington, New Zealand in 2021Ī Van Gogh immersive experience is any of a number of real-life or virtual reality (VR) exhibits of Vincent van Gogh's paintings. 12/1/2023 0 Comments Equal pay day march 24'In March of 2020 over 52 of domestic workers. It’s time for us to close the gap and ensure that women receive equal pay for equal work. Carolyn Maloney held a hearing on Equal Pay Day, examining the gender pay gap and the disproportionate inequalities women of color face. With a high-end salary range, women are able to base their salary negotiations on an industry standard rather than their historical salaries which are often artificially lower from years of inequitable treatment in the workforce. 6380, which requires employers to provide job applicants and employees with salary ranges for vacant positions. This session we are working to address income inequality through a number of legislative proposals, including H.B. But, if we don’t do our part for working women in this moment of COVID-19 crisis, we will revert back decades in terms of women’s fair pay. Over the years, Connecticut has taken significant steps to address the wage inequality, most notability the recent passage and implementation of paid family medical leave was a significant step for women’s ability to stay in the workforce. In Connecticut and nationwide, the wage gap still exists due to gender and racial discrimination, women’s overrepresentation in low-wage jobs and underrepresentation in high-paying jobs, and economic barriers with caregiving responsibilities. The high unemployment rate during the pandemic is expected to only exacerbate these disparities. The gap increases in severity for minority women. This wage gap exists regardless of where women live, what industry they work in or their education level. In 2020, a woman in Connecticut earned only $0.83 for every dollar that a white, non-Hispanic man made. Learn more about Honouring Zelda D’Aprano.Today is Equal Pay Day-a day that marks the point at which it takes a woman on average to earn what her male counterpart did the year before.Read and download Our equal state: Victoria’s gender equality strategy and action plan 2023–2027.One of its core focus areas is ensuring the economic rights and opportunities of Victorians are not limited by gender. On Thursday 24 August, we launched Our equal state: Victoria’s gender equality strategy and action plan 2023–2027. Sculptor Jennifer Mann created the work from the historic photograph.Īround the base is a quote from Zelda that reads: “Today it was me, tomorrow there will be two of us, the next day there will be three and it will go on and on and there won’t be any stopping it.”Īs we remember Zelda’s campaign and note the Equal Pay Day figures, the Victorian Government continues working towards gender equality. Zelda’s equal pay protest is immortalised in bronze in front of the Victorian Trades Hall in Lygon St as part of the Victorian Women’s Public Art Program. On trams they paid 75 per cent of fares because women were paid just 75 per cent of men’s wages and went on outdoor pub crawls because women weren’t allowed to drink in bars. Together they went on to form the Women’s Action Committee, leading activists campaigning across Melbourne. Ten days later two teachers, Alva Geikie and Thelma Solomon, joined in the protest with Zelda. She chained herself to a government building to protest against a ruling against equal pay for women, holding the iconic sign “No more male and female rates. Melbourne’s own Zelda D’Aprano was campaigning for equal pay for women back in 1969. It’s an issue that society has faced – and women have been fighting against – for many years. Although we are heading in the right direction, that is still an average gap of $253 every week or $13,183 every year.Īt the average rate of pay for women, this is the equivalent of eight weeks additional paid work (56 days). In Victoria the gender pay gap is 12.9 per cent (down from 13.5 per cent). In 2020, women earned 83 cents to every dollar earned by men. That’s earlier than it’s ever been since its inception in 1996. This year’s national gender pay gap is 13 per cent, a decrease of 0.3 per cent over the last six months. Equal Pay Day timed to represent how far into the year women must work to equal what men earned the previous year is on March 15 this year. It spells out the stark reality of the gender pay gap that women work 56 days a year for free.Įvery year, the Workplace Gender Equality Agency (WGEA) calculates the grim statistic using Australian Bureau of Statistics average weekly earnings data. Friday 25 August is Equal Pay Day - the number of extra days after the end of financial year that it takes women to earn the same pay as men. Originated by the National Committee on Pay Equity, Equal Pay Day is meant to bring awareness to how far into the year. 12/1/2023 0 Comments Acronis true image hd cloneYou need to choose whether you wish to save online or locally. However, you must note that the app does not allow you to choose both options simultaneously. The local backup software is identical to the online version. More, unlike iDrive, wherein you can do so as a bonus, Acronis True Image offers it as one of its core features. If you wish to keep a copy of your entire drive, this app can do it.īut what will impress you the most is that it gives you the option to save your data locally. Acronis True Image can back up even your system files and applications. Some online backup services are limited to media and document files only. More it offers nearly unlimited storage, so if you have thousands of photos, videos, or music files, you can save all of them. It backs up all of the personal files on your computer to the cloud and keeps them from floods or fires. Here enter true online backup services, such as Acronis True Image. However, as good as they may sound, using your cloud syncing providers to backup all your files may cost too much. These services offer cloud-based storage that copies a set of files or folders while letting you share some files with your contact. You might be familiar with cloud syncing platforms, such as Dropbox, Google Drive, and iCloud. It comes with a wide range of options and features perfect for hardcore PC users. It offers a robust solution for protecting files and disk images using cloud storage. Acronis True Image is one of the best computer backups available today. That is why it is a good thing that there are backup tools available for download. But what makes it devastating is the fact that we sometimes cannot prevent data loss from happening. Losing a file on your computer can be annoying, especially if it is important. |
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