11/30/2023 0 Comments Neuron dendrite analysis![]() In order to get insight into this process, we severed both axon and dendrites of PVD neuron in C. However, little knowledge is available on the mechanism of dendrite regeneration since the study of Cajal. The information receiving neurites, namely dendrites, are also vulnerable to physical insult during stroke and trauma. The knowledge of the repair of injured neural circuits comes from the study of the regeneration of injured axons. This work describes a novel regulatory mechanism of dendrite regeneration and provides a framework for understanding the cellular mechanism of dendrite regeneration using PVD neuron as a model system. Additionally, the function of CED-10 in epidermal cell is critical for post-dendrotomy fusion phenomena. Surprisingly, we found that the RAC GTPase, CED-10 and its upstream GEF, TIAM-1 play a cell-autonomous role in dendrite regeneration. ![]() We found that neither dendrite regrowth nor fusion was affected by the axon injury pathway molecules. We tested the roles of the major axon regeneration signalling hubs such as DLK-1-RPM-1, cAMP elevation, let-7 miRNA, AKT-1, Phosphatidylserine (PS) exposure/PS in dendrite regeneration. Axonal injury causes a retraction of the severed end followed by a Dual leucine zipper kinase-1 (DLK-1) dependent regrowth from the severed end. We quantified the regeneration pattern into three aspects–territory length, number of branches, and fusion phenomena. This was accompanied by reconnection between the proximal and distal dendrites, and fusion among the higher-order branches as reported before. After severing the primary dendrites near the cell body, we observed sprouting of new branches from the proximal site within 6 hours, which regrew further with time in an unstereotyped manner. Using femtosecond laser, we severed the primary dendrites and axon of this neuron. elegans with stereotyped branched dendrites. To understand the mechanisms of dendrite regeneration, we used the PVD neurons in C. Although several molecular pathways of axon regeneration are identified, our knowledge of dendrite regeneration is limited. Neurons are vulnerable to physical insults, which compromise the integrity of both dendrites and axons.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |